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Shruthi Prakash, Anita.P.M, Kusum Paul


Large intergenic noncoding RNAs (lincRNAs) are emerging as key regulators of diverse cellular processes.Determining the function of individual lincRNAs remains a challenge. Recent advances in RNA sequencing (RNA-seq). Here, we presentan integrative NGS approach to define a reference catalog of >3000 human lincRNAs.Our catalog unifies previouslyexisting annotation sources with transcripts we assembled from RNA-seq data collected from RNA-seq reads across 2 tissue and cell types. We have aligned and assembled the reads to the reference genome version 19 (Hg19).We found that lincRNA expression is strikinglytissue-specific compared with coding genes, and that lincRNAs are typically coexpressed with their neighboringgenes, Our integrated, comprehensive, yet conservative reference catalog of humanlincRNAs reveals the global properties of lincRNAs and will facilitate experimental studies and further functionalclassification of these genes.

Keywords: long noncoding RNAs; RNA sequencing; lincRNAs.

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