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Abstract

COMPARATIVE STUDY OF PRULIFLOXACIN BY VALIDATED RPHPLC AND MICROBIAL AGAR ASSAY METHOD USING STUDENT’S T TEST

Sweety Kanolkar, Teja Walke, Yogita Sardessai

ABSTRACT

A simple, accurate and precise RPHPLC method was developed for determination of Prulifloxacin in tablet dosage form. The chromatography was performed on a C18 column. Eluents were monitored by UV detection at 279 nm using the mobile phase Acetonitrile: Phosphate buffer pH 7(80:20). The method was statistically validated for linearity, accuracy, precision. The linearity of prulifloxacin was within the concentration range of 16-24 μg/mL. The limits of detection and quantitation were 0.189 μg/ mL and 0.5751 μg/ mL respectively. Microbial agar assay was chosen as a comparison method for the prulifloxacin determination. Assay of prulifloxacin determined in both methods were compared statistically using student’s t test which confirmed that the two methods are statistically similar and there is no significant difference.

Keywords: prulifloxacin, RPHPLC, microbial agar assay, student’s t test.


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