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Abstract

IN VITRO ANTIOXIDANT ACTIVITY OF NYMPHAEA RUBRA L. RHIZOME

Edison Dalmeida Daffodil and Veerabahu Ramasamy Mohan*

ABSTRACT

The in vitro antioxidant capabilities of methanol extract of Nymphaea
rubra rhizome was evaluated by studying 1,1-diphenyl-2-
picrylhydrazyl (DPPH) radical scavenging activity, hydroxyl radical
scavenging activity, superoxide radical scavenging activity, ABTS
radical cation scavenging activity and reducing power using standard
procedure. Total phenolic content was estimated by folin-ciocalteau
method. Flavonoid was determined by Aluminium chloride method.
The total phenolics and flavonoids in methanol extract were found to
be 0.36 g 100g-1 and 0.67 g 100g-1 respectively. The methanol extract
of rhizome showed potent in vitro antioxidant activities. Among
different concentration tested, methanol extract of N. rubra rhizome at
1000 μg/mL showed potent scavenging activity in all models viz,
DPPH, hydroxyl radical, superoxide and ABTS. The result clearly
Indicates that, methanol extract of N. rubra rhizome is effective in scavenging free radicals
and has the potential to be a powerful antioxidant.

Keywords: Nymphaea rubra, flavonoid, ABTS, reducing power.


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