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Abstract

RP-HPLC METHOD DEVELOPMENT AND VALIDATION OF NORFLOXACIN IN BULK FORM

Sharma Ravi*, Kaur Amandeep, Malhotra Dipali

ABSTRACT

A simple, selective, accurate and precise high-performance liquid chromatographic (HPLC) method for estimation of Norfloxacin in bulk form was developed & validated. The estimation was carried out on Phenomenax luna C-18 (250x4.6 mm, 5μ) column using a mobile phase consisting of 0.03 M potassium dihydrogen phosphate, triethyamine: Acetonitrile (88:12 v/v) of pH 3.8 adjusted with orthophosphoric acid, at a flow rate 1 ml/min. The UV detection was carried out at 280 nm. Method validation was performed as per the ICH guidelines. The method was validated for the precision, accuracy, linearity and robustness.

Keywords: RP-HPLC estimation, Validation and Norfloxacin


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