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Abstract

ASSESSMENT OF DIFFERENT RESTRICTION ENDONUCLEASES: (SAL 1 AND XBA 1) ON PURIFIED CHLAMYDIA PNEUMONIA DNA

Otoikhian C.S.O.*, Okoror, L.E. Ekakitie, A.O.

Novena University, College of Natural and Applied Sciences, Department of Biological Sciences, Delta State, NIGERIA.

ABSTRACT

Two restriction endonucleases (Sal 1 and Xba l) where compared to ascertain if same strain of Chlamydia pneumonia that is responsible for respiratory tract infection of the patients isolated from Delta State of Nigeria. Results revealed that 95 samples (76.61%), where positive while 29 samples (23.38%) were negative using the culture method and staining with giemsa technique. The restriction endonucleases Sal l and Xba 1 cleaved the DNA at different point as evidenced on agrose gel electrophoresis and also the DNA could not be cleaved by both restriction endonucleases indicating strain variation in Chlamydia pneumonia species.

Keywords: DNA, strain, Chlamydia pneumonia.


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