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Abstract

DEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF CEFIXIME TRIHYDRATE AND ORNIDAZOLE IN TABLET DOSAGE FORM

*Smita S. Aher, Dr. R .B. Saudagar.

ABSTRACT

A simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed and validated for the estimation of Cefixime Trihydrate and Ornidazole simultaneously in combined dosage forms. The stationary phase used was precoated silica gel 60F254. The mobile phase used was a mixture of Methanol: Toluene (7:3 v/v) The detection of spots was carried out at 301 nm. The method was validated in terms of linearity, accuracy, precision,LOD andLOQ. The calibration curvewas found to be linear between 60to 160 ng/spot for Cefixime Trihydrate and 150 to 400 ng/spot for Ornidazoleand Itopride is used as internal Standard. The proposed method can be successfully used to determine the drug content of marketed formulation.

Keywords: Simultaneous estimation, HPTLC ,Validation , Cefixime, Trihydrate (CEF), Ornidazole(ORD), Itopride(ITO).


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