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Abstract

DEVELOPMENT AND VALIDATION OF A ULTRA PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR SIMULTANEOUS DETERMINATION OF HYDROCHLOROTHAIZIDE AND ALMODIPINE IN FORMULATIONS

E.Sasikiran Goud*, Dr. V. Krishna Reddy, N. Suresh Babu

ABSTRACT

Aim of the present work was to develop a simple, shorter and effective UPLC method with dual mode, 270 for hydrochlorothiazide and 237 for amlodipine and subsequent validation for the simultaneous determination. The effective on achieved on Zorbax RP-8, 50x4.6 mm, 3.5μ column. The mobile phase is composition of buffer and acetonitrile in the ratio of 64: 34v/v . The buffer was prepared by dissolving the 2g of sodium perchlorate and 1mL of triethyl amine in 1000mL of water and adjusts pH 3.6 with orthophosperic acid. The flow rate of mobile phase was 1.2mL/min with injection volume 2μL. The relative standard deviation for five injections was observed to 0.1 for hydrochlorothiazide and 0.1 for amlodipine. The observed result shows that the method was rapid, precise, accurate and simple. The method was validated as per ICH guidelines.

Keywords: Hydrochlorothiazide, Amlodipine, UPLC, Method development and Validation.


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