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Abstract

IN VIVO AND IN VITRO BIOCHEMICAL ESTIMATION OF METABOLITES FROM TWO MEDICINALLY IMPORTANT PLANTS VIZ. INDIGOFERA CORDIFOLIA AND INDIGOFERA LINNAEI

Shipika Upman, Renu Sarin* and Jasvinder Kaur Chahal

ABSTRACT

Since ancient times medicinal plants have been virtually used in all cultures as a source of medicines. Primary metabolites obtained from plants for their pharmacological assay. Wild plants are the vast source of innumerable therapeutic agents representing molecular diversity engineered by nature. This research article shows the composition of primary metabolites from two plants species of family Fabaceae. Two medicinally important plants were selected for the present study viz. Indigofera cordifolia and Indigofera linnaei. The primary metabolites (total soluble sugar, starch, lipids, proteins and phenol) of Indigofera cordifolia and Indigofera linnaei have been estimated from different plant parts viz., leaves, stem, root and callus. The observations of the study showed maximum amount of total soluble sugar and starch in roots (58.0±0.21 mg/g.d.w) and stem (56.33±0.47 mg/g.d.w) of I. cordifolia respectively. Proteins in the stem (48.3±1.24 mg/g.d.w), lipids (75.67 mg/g.d.w) in leaf explant of I. cordifolia but phenol content was highest(50.0±0.21 mg/g.d.w) in stem of I. linnaei in comparison to other parts investigated.

Keywords: Indigofera cordifolia, Indigofera linnaei, Primary metabolites-total soluble sugar, starch, lipids, proteins and phenol.


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