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Dr.RbabOmran, Khaled Y Alzamilyand ,*Dr. Ibrahim M S Shnawa


The vibrio species and the vibroid enteric isolates were found as Vibrio fluvialis andAeromonashydrophila though using API20E identification system. The to be total DNApreparations were separated from both of the organisms on agarose gel electrophoriesisand confirmed through the use of UV trsnsillumination. Such prepared and purifiedDNAwere amplified by PCR and allowed to pair with primers of four virulence genes as; Vhf,hupO ,tox and VhpfA for V .fluvialis and the primers of the genes Aer, hyl for A.hydrophila.Only Vhf and tox genes were positive in V.fluvialis and the genes Aer ,hyl were positive inA.hydrophila. Thus, PCR can be of use for the diagnosis for both of these organisms fromclinical samples,this will reduce labor and time needed for diagnosis.The authors were of theopinion holds that,four virulence genes from each of these organisms may served as abattery useful in molecular diagnosis of these disease agents

Keywords: Haemolysin ,Aerolysin ,Virulence genes ,attenuation ,expression ,exotoxins.

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