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Abstract

ASSESSMENT OF GENETIC DIVERSITY IN WATERMELON (CITRULLUSLANATUS (THUNB.) BY USING SDS-PAGETHROUGH GENETIC ANALYSIS SOFTWARE

Rajesh Goud Gajula*, Dr. Shailima RD Vardhini, Dr. Sujatha Edupuganti, G.Raghavendar

ABSTRACT

Seed storage Protein profiles of five varieties of CitrullusLanatus were
analysed by sodium dodecyl sulphate polyacrylamide gel
electrophoresis (SDS-PAGE). Total seed storage protein of Citrullus
Lanatus were resolved on 10% SDS Polyarylamide gels showed
variations in their banding pattern. The dendrogram constructed by
NTSys PC software. The SAHN clustering based on UPGMA
algorithms has put the all accession into different cluster groups. The
Jaccord’s coefficient also calculated. Maharaja Variety and Super
dragon verity has close genetic relation. Shahanshah and Maharaja
have highest variation among five cultivars.Jaccard’s similarity shows
that Maharaja and Shahanshah are 55% similar which is less similarity in all cultivars,
Maharaja and Super Dragon are 73% similar, Maharaja and Durgesh has 73% similarity,
Super Dragon and Durgesh are 71% similar. More work is needed to identify the specific
genes in the cultivars, quantitative trait loci (QTL) works are carrying out to identify and
correlate the genes for different traits using RAPD Technique.

Keywords: Citrullus Lanatus, SDS-PAGE, Storage protein, Genetic diversity, Electrophoresis, NTSys PC, SAHN, UPUGMA, Jaccord’s Coefficient.


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