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Abstract

THE POSTCHALLEMGE IMMUNE PROTECTION MEDIATED BY SMOOTH LIPOPOLYSACCHRIDE OF VIBRIO FLUVIALIS IN A MOUES MODEL

Dr Ibrahim M S Shnawa*, ALZamily K. Y., Dr Rabab Omran

ABSTRACT

From V.fluvialis smooth form culture a lipopolysacchride LPS was prepared and purified into the limits of partial purity as F1 and F2.These F1 and F2 were assessed for mitogenicity, immunogenicity and immune protectivity in a mouse model.They were mitogenic through skin induration and bone marrow lymphocyte blatogenicity, rise up of V.fluvialis specific antibody titres, cytokine activator for IL4,IL6,and TNF alpha causing postchallenge cytokine imbalance between proinflammatory IL6 and anti-inflammatory IL4 and immune protective via survivors record. Thus, V. fluvialis SLPS F1&F2 were mitogenic, immunogenic and postchallenge immune protective in mouse model.

Keywords: Vibrio, Smooth, culture, LPS, mitogenicity, immunogenicity Postchallenge, immune protection.


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