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Abstract

PRODUCTION AND CHARACTERIZATION OF NAPHTHOQUINONE PIGMENT FROM FUSARIUM MONILIFORME MTCC6985

Premalatha B., Stanly Pradeep F., Pradeep B. V. and Palaniswamy, M*

Department of Microbiology, Karpagam University, Coimbatore 641 021, Tamilnadu, India.

ABSTRACT

Production and characterization of naphthoquinone pigment by the fungus Fusarium moniliforme MTCC6985 was investigated in this study. Cultivation conditions, including various medium, temperature, pH of the medium, time of incubation, carbon source, nitrogen source and mineral salts were optimized to improve the yield of total naphthoquinones in shake flask culture of Fusarium moniliforme MTCC6985. The highest yield of total naphthoquinones was obtained in Potato dextrose broth (PDB) supplemented with 2% glucose, yeast extract, potassium dihydrogen phosphate with a pH of 5.5 at 28C. The purified and crude pigment extracted from the biomass was analyzed by TLC, UV absorption spectrum and FTIR spectroscopy.

Keywords: Naphthoquinone, Fusarium moniliforme MTCC6985, Potato dextrose broth.


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