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Abstract

EVALUATION OF ANTIBACTERIAL ACTIVITY OF PHOSPHATE ESTERS BY WELL DIFFUSION METHOD

Mithilesh Kumari Gupta, S. K. Jadhav, S. A. Bhoite*

ABSTRACT

Resistance in some pathogenic bacterial strains to conventional
antibiotics has initiated to search for effective treatments against
microorganisms. In this context antibacterial activity of mono-6-
chloro-2,4-dinitroaniline phosphate and di-2-methyl-5-nitroaniline
phosphate were studied by determining the zone of inhibition of
bacterial growth against four pathogenic bacterial strains
Staphylococcus aureus MTCC 3160, Klebsiella oxytoca ATCC 13182,
Bacillus subtilis BAB 2437 and Bacillus licheniformis MS 17.
Different concentrations of test compounds were applied against
bacterial strains to examine their antibacterial activity by using well
diffusion method. Gentamycin and water were taken as a positive and
negative control to test the bioactivity of phosphate esters. Zone of inhibition was observed
and measured in mm. Minimum inhibitory concentration (MIC) of phosphate esters against
pathogenic bacterial strains was also determined.

Keywords: Mono-6-chloro-2,4-dinitroaniline phosphate, di-2-methyl-5-nitroaniline phosphate, antibacterial activity, well diffusion method, zone of inhibition, MIC.


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