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Abstract

EVALUATION OF PHYTOCHEMICAL CONSTITUENTS BY GAS CHROMATOGRAPHY-MASS SPECTROSCOPY & HPTLC OF CALOTROPIS PROCERA

B. Dwivedi, A. Singh*, S. Mishra, R. Singha, P.Pant , L.K.Thakur, M.M.Padhi

ABSTRACT

In the present study the major phyto-constituents of leaves hexane extract of Calotropis procera investigated qualitatively & quantitatively by GC-MS. A optimize HPTLC profile of leaves extract has developed to resolved and screened the phyto compounds appeared in UV light and after derivatization. In HPTLC analysis seven bands in 254nm, twelve bands in 366nm & five bands after derivatization were observed at different Rf values. Twelve major phyto compounds were identified and estimated by GC-MS analysis. The result obtained by GC-MS study revealed that in hexane extract of leaves, highest peak area % of 25.22 was obtained by Ergost-5-en-3-ol (C28H48O) at retention time of 45.020 and the lowest peak area % of 0.24 was obtained by 9 octadecenoic acid 9-Octadecenoic acid (Z)- (C18H34O2) at retention time of 17.353. This study summarizes the information concerning the phyto-constituents present in hexane leaf extract which may be responsible for various therapeutically effects.

Keywords: C. procera, Phytochemistry, Hydrocarbons, HPTLC Methods, GC-MS, Metabolites, Leaves.


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