WJPR Citation New

  All Since 2011
 Citation  2903  2393
 h-index  27  24
 i10-index  68  60

Login

Best Paper Awards

World Journal of Pharmaceutical Research (WJPR) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.
            Best Paper Award :
Dr. Muhammad Baqir MR Fakhrildin
Download Article: Click Here

Search

Track Your Article

Abstract

STUDY ON RADICAL SCAVENGING AND GASTROPROTECTIVE EFFECTS OF ETHANOLIC STEM-BARK EXTRACT OF CARPOLOBIA LUTEA IN RODENTS

Lucky Legbosi Nwidu*, Jonah Sydney Aprioku, Fred Horsfal Guluye

ABSTRACT

This is a study of the antioxidant activity and gastroprotective effects of Carpolobia lutea ethanolic stem-bark extract (CL ESE) used as antiulcer in ethnomedicine. Experimentally induced gastric ulceration was achieved using acute models such as indomethacin, ethanol, stress and serotonin. Amino acid analysis was by cation-exchange chromatography using automated amino acid analyser. Antioxidant potential was obtained by spectrophotometric assay using 2, 2- Diphenyl-1-picrylhydrazyl DPPH. The finger print of ESE was revealed by Jasco (Tokyo, Japan) HPLC and active compounds by phytochemical screening using standard procedure. The calculated LD50 is 866.025 mg/kg (i.p) using Lock's Method. In the indomethacin model, 43.3, 86.6 and 173.2 mg/kg revealed ulcer inhibition rate of 51 %, 65 % and 76 % respectively (p > 0.05-0.01) when compared with the control (p<0.05) while the pure drugs, Cimetidine (100 mg/kg) gave ulcer inhibition rate of 74 % (p<0.01). In the ethanol model, 43.3, 86.6 and 173.2 mg/kg of the extract gave ulcer inhibition of 20 %, 28 % and 40 % respectively; while cimetidine produced 36 % ulcer inhibition (p>0.05). But combination of both 86.6 mg/kg of the extract and Cimetidine 100 mg/kg revealed the highest ulcer inhibition rate of 90% (p<0.001). The reaction to DPPH was less than 10%. The HPLC fingerprint of ESE revealed UV spectra of biomolecules. High concentration of Tyrosine, Glutamine, Methionine, glutamine, Phenylalanine and Arginine were observed in the CL ESE. Phytochemical screening revealed presence of saponins, tannins, polyphenols and glycosides. These investigations indicate presence of bioactive and elemental substances which in part may play significant role in gastric ulcer management. This investigation validates the use of stem-bark of C. lutea in illicit gin (akpatashi) among the Effiks in Nigeria as antiulcer.

Keywords: Carpolobia lutea, stem-bark extract, antiulcerogenic, amino acid and antioxidant profile.


[Full Text Article]

Call for Paper

World Journal of Pharmaceutical Research (WJPR)
Read More

Email & SMS Alert

World Journal of Pharmaceutical Research (WJPR)
Read More

Article Statistics

World Journal of Pharmaceutical Research (WJPR)
Read More

Online Submission

World Journal of Pharmaceutical Research (WJPR)
Read More