WJPR Citation New

  All Since 2011
 Citation  2903  2393
 h-index  27  24
 i10-index  68  60

Login

Best Paper Awards

World Journal of Pharmaceutical Research (WJPR) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.
            Best Paper Award :
Dr. Muhammad Baqir MR Fakhrildin
Download Article: Click Here

Search

Track Your Article

Abstract

GC-MS ANALYSIS OF PHYTOCONSTITUENTS AND CONCURRENT DETERMINATION OF FLAVONOIDS BY HPLC IN ETHANOLIC LEAF EXTRACT OF BLEPHARIS MADERASPATENSIS (L) B. HEYNE EX ROTH

M. Suriyavathana* and S. Indupriya

ABSTRACT

Objectives: To elucidate the phytochemical constituents using GC-MS and a sensitive HPLC method has been developed for the concurrent determination of five major flavonoids in ethanolic leaves extract of Blepharis maderaspatensis. Methods: The shade-dried leaves of B. maderaspatensis were extracted with ethanol, the concentrated ethanolic extracts were further subjected to GC-MS and HPLC. Results: The GC-MS analyses determined the presence of 17 different phytochemical compounds in the ethanolic leaves extract of B. maderaspatensis. The phytoconsituents compounds were found in the mass spectra was matched with the National Institute of Standards and Technology (NIST) library. The major phytoconstituents are 9- Eicosyne (23.64%), Sqalene (11.51%), Phytol (8.40%), 3,4-Dihhdro-3,5,8-trimethyl-3- (4,8,12trimethyltridecyl)-(2H)-benzopyran-6-acetate (8.07%), 3,7,11,15 Trimethyl-2- hexadecen-1-ol (7.41%) and Cholestan-3-ol, 2 methylene,(3á,5à) (7.24%). Mass spectra of the compounds found in the extract were matched with the National Institute of Standards and Technology (NIST) library. The Phytochemical profile of five flavonoids contents in the ethanolic leaf extract of B. maderaspatensis are Gallic acid (16.0 μg/g), Caffeic acid (0.1 μg/g), Rutin (0.1 μg/g), Quercetin (0.2 μg/g) and Ferulic acid (0.01 μg/g). Conclusions: This is the first report of documentation of active constituents from leaves of B. maderaspatensis. The results of the present study reveal that the leaves of B. maderaspatensis indulge effective potential bioactive compounds, which may be leads to the formulation of new drugs to treat various diseases.

Keywords: Blepharis maderaspatensis (L) B. Heyne ex Roth Phytochemicals Flavonoids.


[Full Text Article]

Call for Paper

World Journal of Pharmaceutical Research (WJPR)
Read More

Email & SMS Alert

World Journal of Pharmaceutical Research (WJPR)
Read More

Article Statistics

World Journal of Pharmaceutical Research (WJPR)
Read More

Online Submission

World Journal of Pharmaceutical Research (WJPR)
Read More