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Abstract

CHROMATOGRAPHIC FINGERPRINT ANALYSIS OF STEROLS IN OROXYLUM INDICUM (L) VENT BY HPTLC TECHNIQUE

Saraf Aparna* K. Srilatha Srinivas

ABSTRACT

The study was carried out with an aim to determine the chemical
profile of sterols from leaf , stem and roots of the medicinally
important plant Oroxylum indicum (L) Vent collected from two
different geographical regions of India viz., Western Ghats and North
East. Preliminary phytochemical screening was done followed by
extraction of sterols and then by HPTLC studies. Toluene: methanol
(9:1) was used as mobile phase for the separation of sterols. β
Sitosterol was used as a marker compounds and was detected in all the
plant parts collected from both the regions. A good seperation of
sterols was observed at 540nm. The study revealed diverse forms of
sterols in large number in the root, stem and leaf of Oroxylum indicum (L) Vent. from both
the regions. It can be concluded that HPTLC fingerprint analysis of root, stem and leaf
extract of Oroxylum indicum (L) Vent can be used as a diagnostic tool for the correct
identification of the plant and it is useful as a phytochemical marker. These separated sterols
in addition of being developed directly as drugs can also served as prototype drug molecules
known as “Lead Compounds” and as pharmacological probes to help better understand
biochemical and physiological mechanisms.

Keywords: Oroxylum indicum (L) Vent, sterols, HPTLC fingerprint.


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