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Abstract

MOLECULAR CHARACTERIZATION OF JASMINE SPECIES BY RAPD MOLECULAR MARKERS

Sushant Shekhar and Prasad M.P*

ABSTRACT

In the following study genomic DNA was extracted from eight
Jasmine species. DNA fingerprinting was performed by the random
amplified polymorphic DNA (RAPD)-PCR. RAPD has been one of the
most commonly used molecular techniques to develop DNA markers
since it does not require prior knowledge of a DNA sequence. RAPDPCR
produced a spectrum of amplification products which are
characteristics of the selected Jasmine DNA. From the DNA
fingerprinting, Dendrogram was constructed and genetic similarity
matrix were estimated which revealed variations between selected
species of Jasmine. A total of 15 random primers were used for conducting the RAPD
analysis. The primers were OPZ5, OPZ6, OPZ7, OPZ8, OPZ9, OPZ10, OPZ11, OPZ12,
OPZ13, OPZ14, OPZ15, OPZ18, OPZ19, OPZ20, OPZ21 and OPZ22. Of these selected
random primers OPZ8, OPZ9, OPZ10 produced clear banding patterns which were used for
constructing Dendrogram. OPZ8 produced a total 58 bands ranging from 7-11, OPZ9
produced a total 91 bands ranging from 10-13 and OPZ 10 produced a total 49 bands ranging
from 5-8 for eight jasmine samples. Most of the bands were monomorphic with some
polymorphic bands which can be used for marker development for these jasmine species. The
described approach holds great promise for genetic diversity polymorphism, cultivar
characterization and genetic population conservation of Jasmine species.

Keywords: Jasmine, DNA fingerprinting, RAPD, Genetic Diversity.


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