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Abstract

COMPARATIVE PHYTOCHEMICAL STUDY OF STEM BARK VERSUS SMALL BRANCHES OF FICUS RELIGIOSA LINN USING HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHIC ULTRA VIOLET DETECTION METHOD

S. C. Verma*, E. Vashishth, S. Subhani, R. Singh, P. Pant and M. M. Padhi

ABSTRACT

Ficus religiosa Linn, family Moraceae is an important medicinal plant and immensely used in the Indian System of Medicine to cure human diseases. Stem bark of F. religiosa has been reported for various medicinal properties such as an astringent, aphrodisiac, antiinflammatory, antiseptic, antidote, antibacterial against Staphylococcus aureus and Escherichia coli, and in bleeding, bone fracture, burns, cooling, diabetes, diarrhoea, dysentery, gonorrhea, gastrohelcosis, haemorrhoids and paralysis and many more. F. religiosa is commonly known as Udamber in India. Chemo-profiling screening of two parts of F. religiosa plants revealed variations in phytochemicals within stem bark and small branches. The unique properties of the chromatographic fingerprint were validated by analyzing stem bark and small branches of F. religiosa. Our results revealed that the chromatographic Finger print combined with similarity measurement could efficiently identify and distinguish F. religiosa from the other investigated Ficus species. In this paper a new, simple method is proposed in which the HPTLC-UV pattern of the extracts of stem bark and small branches of F. religiosa content is used for comparison of phytochemical present in both drug. The method can also be used for identification of different F. religiosa species. The proposed method uses cold- extraction then clean-up by solid-phase extraction before chromatographic analysis. The results revealed that the retention factor (Rf) of F. religiosa stem bark and small branches furnished a specific HPTLC chromatogram fingerprint which might be helpful for quality assurance and detection of adulteration of crude extracts.

Keywords: F. religiosa Linn, HPTLC–UV detection, phytochemical Fingerprint profiling analysis.


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