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Abstract

ACUTE TOXICITY (LETHAL DOSE 50 CALCULATION) AND HISTOPATHOLOGICAL EFFECTS OF METHANOLIC EXTRACT OF BERBERIS VULGARIS IN MICE

Muhammad Ahmed*, Aisha Azmat

ABSTRACT

The arithmetic method of Karber and histopathology were used to study the acute toxicological effects of Methanolic extract of Berberis vulgaris root pulp (BRPM) on albino mice. The study involved different doses (10, 100, 500 and 1000mg/Kg) of the extract by intraperitoneal administration to groups of mice. Signs of toxicity and possible death of animals were monitored for 24 hrs to ascertain the median lethal dose (LD50) of the extract. At the end study, all the animals in all the dose groups were sacrificed and the internal organbody were compared with values from the control group. The LD50 was found to be 750 mg/Kg body weight upon intraperitoneal administration in mice. The histopathological examination indicated that the at the dose of 1000mg/Kg, extract induced several histopathological changes in the liver of mice such as necrosis of some hepatocytes, edema, scattered bile canaliculi and damage in septa between lobes of liver. The obtained LD 50 values classify the BRPM (i.p.) as slightly toxic according to Hodge and sterner toxicity scale.

Keywords: Acute toxicity, Berberis vulgaris, Histopathological effects, Lethal dose (LD), Median Lethal dose (LD50).


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