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Abstract

SCREENING FOR SIDEROPHORE AND AMMONIA PRODUCTION FROM RHIZOSPHERE ISOLATES OF ORYZA SATIVA

Ritesh Singh and M.P. Prasad*

ABSTRACT

The Rhizosphere is biologically and chemically highly diverse, complex and dynamic interaction occurs between plant roots, soil (micro) biota and the physicochemical conditions of the soil. The autotrophic plant partner is providing substrate and energy flow into the rhizosphere and gets in return essentials for its development and growth: nutrients, minerals and water. Heterotrophic soil biota usually are limited in the supply of carbon and energy and thus a complex sequence of responses are initiated, which in due course also influence the plants. In the present study the microorganisms were isolated from the rhizosphere soil of rice from nine different locations in and around Bangalore to understand the diversity of the microorganisms, interaction and to determine the siderophore activity and production of ammonia of the isolates. A total of 116 micro organisms were isolated of which 110 were bacterial isolates and 6 Actinomycetes. 53 bacterial isolates found to be morphologically different were used for the current study. 6 isolates out of the 53 microorganisms showed positive results for the production of siderophore by forming orange yellow halo zones on CAS agar media plate. R-19 isolate showed the highest zone and was considered as the highest producer of siderophore. Whereas 36 isolates showed positive for ammonia production out of which 6 isolates showed maximum production and 12 isolates with fairly good production.

Keywords: Rhizosphere soil, siderophore, Rice, CAS, Actinomycetes.


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