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Abstract

SCREENING FOR SOME MUTATIONS IN MITOCHONDRIAL ND1 GENE ASSOCIATED WITH T2DM IN IRAQI POPULATION

Ismail A. Abdul-Hassan* and Zahraa L. Hameed

ABSTRACT

This study was conducted to investigate the role of mitochondrial ND1 gene in the incidence of type 2 diabetes mellitus (T2DM). Fifty T2DM patients and thirty apparently healthy individuals (control) were used in this study. Blood samples were collected at National Center for Diabetes treatment and Research- College of Medicine ,University of Al-Mustansiryah from both gender (42 male and 38 female ), aged between 50-70 years. Statistical analysis of the questioner form showed that most of the T2DM patients are overweight with body mass index (BMI) value ≥ 25kg. Conventional polymerase chain reaction was used for identification and amplifying the specific DNA fragment and then the PCR product incubate with Hae III restriction enzyme, then loaded by agarose gel electrophoresis. The G3316A mutation in the NADH dehydrogenase (ND1) gene was confirmed by sequencing. Results revealed that G3316A mutation in the ND1 gene was not associated with type 2diabetes mellitus risk in Iraqi patients. In addition, the sequence analysis indicated the presence of m.3480A˃G in 4 control individuals; m.3505A˃G in one T2DM patient; m. 3429C˃T in 2 T2DM patients and m.3713delT in 7 control individuals and 10 T2DM patients. There was no correlation between mitochondrial ND1 gene mutations studied herein and type 2 diabetes mellitus in Iraqi patients.

Keywords: mitochondrial ND1 gene, mutation, T2DM.


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