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Mohammad Sakim Majumder, Mohammad Nurul Amin, Shimul Banik , Md. Mizanur Rahman Moghal*, Md. Ibrahim, K.M. Rahat Maruf Jitu, Md. Emdadul Hasan Mukul, Rajib Chandra Da


The present study was conducted to detect possible chemicals (phytoconstituents), and investigate antioxidant, antimicrobial and thrombolytic activities of the extract of a medicinal herb, Polygonum viscosum (leaves). Phytochemical screening was carried out using the standard test methods of different chemical group. For investigating the antioxidant activity, two complementary test methods namely DPPH free radical scavenging assay and total phenolic content determination were carried out. For the evaluation of in vitro antimicrobial activity, disc diffusion method, and to determine the thrombolytic activity, the method of Prasad et al., 2007 with minor modifications were used.The extracts were a rich source of phytochemicals. In DPPH free radical scavenging test, the the Chloroform soluble fraction showed the highest free radical scavenging activity with IC50 value 15.09μg/ml while compared to that of the reference standards ascorbic acid. Polygonum viscosum was also found as a good source of total phenolic contents. Morever, the extracts revealed good antimicrobial activity at the concentration of 400 μg/disc. By comparing with the negative control the mean clot lysis % was significant (p value <0.0009). Therefore, further studies are suggested to determine the active compounds responsible for the biological activities of the plant extracts.

Keywords: Phytochemical, antioxidant, antimicrobial, thrombolytic, Polygonum viscosum.

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