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Abstract

DEVELOPMENT AND VALIDATION OF A UV SPECTROSCOPIC METHOD FOR ANALYSIS OF TIZANIDINE HYDROCHLORIDE

K.E Pravallika*, P.Ravi, D.Lalitha Kumari, Hemavathi and M.Bhavya

ABSTRACT

The method has been developed and validated for the assay of Tizanidine hydrochloride using distilled water. The λmax (absorption maxima) of the drug was found to be 228 nm. Linear response was observed in the range of 4-24μg/ml with a regression coefficient of 0.999.Furthermore various validation parameters as per ICH Q2B guideline were tested and found accordingly. The percent purity (99.09 %) and % Recovery at 50, 100 and 150 % were found to be 99.93%, 101.77%, 100.07% respectively for marketed Tizanidine hydrochloride tablets. Developed method was a less toxic, cheap, eco-friendly but equally sensitive spectroscopic method for quantitative determination of Tizanidine hydrochloride for regular quality control purpose in laboratories.

Keywords: Spectrophotometry, Tizanidine hydrochloride, Accuracy, precision.


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