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Anamica Das*, Gopinath Chattopadhyay, Debosmita Kundu, Raju Mandal, Prasun Roy, Dr. Arup Kumar Mitra


The bacteria present in dye effluents often show diverse features including different biochemical properties, ability to grow in a variety of media, tolerance towards varying physical conditions and ability to degrade dye. It plays an important role in the toxic dye remediation program and may be used as an effective organism for removing the toxic dye present in textile and other associated industrial effluent. The present investigation was carried out to isolate dye degrading bacterial species from an untreated textile mill effluent. On characterization of the yellow coloured sample, the bacterial isolate was screened for its ability to degrade azo dye (highly toxic metanil yellow dye degradation). A simple bioassay was carried out by serial diluting and pours plating the dye in nutrient medium. A single strain of bacteria was taken into account for further studies including colony Morphology, Biochemical tests and enzyme assay. The bacterium was identified as Bacillus cereus PM76 on 16S rRNA identification. On performing the enzyme assay experiment, it was found that the bacteria is capable of producing mainly azo-reductase along with catechol 1,2-dioxygenase and peroxidase. The total hydrocarbon present was scanned by Reverse Phase OD S2 column.

Keywords: Dye degradation, Citrate Agar, Nitrate utilization test, Azo dye, Bioremediation.

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