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Abstract

ESBL, MBL AND AmpC DETECTION IN MULTIDRUG RESISTANT PSEUDOMONAS AERUGINOSA (MDRPA) AND PANDRUG RESISTANT Pseudomonas aeruginosa (PDRPA) ISOLATED IN TERTIARY CARE HOSPITAL

Vijay Mane*, A D Urhekar and Nitin Goel Insan

ABSTRACT

Pseudomonas aeruginosa is a leading cause of nosocomial infection.
The rise of antibiotic emergence of resistance may vary with different
antibiotic treatments. A variety of ß-lactamases which include ESBLs,
AmpC ß-lactamases and metallo-ß-lactamases, have emerged as the
most worrisome mechanism of resistance among the gram negative
bacteria, which pose a therapeutic challenge to the health care settings.
Materials and Methods: A total of 190 clinical isolates of
Pseudomonas aeruginosa were studied. Detection of AmpC betalactamase
was performed by three-dimensional method, whereas
detection of ESBL was done by the combined disk diffusion method as
per Clinical and Laboratory Standards Institute (CLSI) guidelines and
MBL were detected by the combined disc diffusion test.

Keywords: .


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