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Abstract

IMPURITY PROFILING OF OXYBENZONE BY RP-HPLC METHOD

Md. Azheruddin*, Akiful Haque, G Shravan Kumar, Vasudha Bakshi, B Sriram Murthy.

ABSTRACT

A simple, accurate, economical and reproducible reverse phase high performance liquid chromatographic (RP-HPLC) method was developed for the determination of Ben-1, Benzophenone, Benzyl trichloride and Resorsinol in Oxybenzone. The separation was achieved on a kromosil C18 column (150 × 4.6 mm i.d, particle size of 5 μ) using a mixture of Formic Acid(0.2%) and acetonitrile in the ratio of 80:20 %v/v as mobile phase in an isocratic elution mode, at a flow rate of 1ml/min. The detection was monitored at 250nm for Ben-1, Benzophenone, Benzyl trichloride and Resorsinol. The retention time of Ben-1, Benzophenone, Resorsinol and Benzyl trichloride were found to be 15.738, 18.682, 2.747 and 8.328 mins respectively. The method was validated for Recovery studies. Method was successfully applied for the determination of Ben-1, Benzophenone, Benzyl trichloride and Resorsinol in Oxybenzone sample.

Keywords: Ben-1, Benzophenone, Benzyl trichloride, Resorsinol Oxybenzone, RPHPLC method.


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