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Abstract

DEVELOPMENT AND VALIDATION OF NEW ANALYTICAL METHOD FOR OLMESATRON BY RP-HPLC

T. Gopi Raju* and Dr.K Vanitha Prakash

ABSTRACT

Olmesarton is an angiotensin antagonist used in treatment of hypertension. The goal of present work was planned to develop accurate, precise, specific, and reproducible. A rapid, specific stability indicating reverse phase high performance liquid chromatography method has been developed and validated for olmesarton. The HPLC analysis used a reversed phase C18 (150 x 4.6mm, 5μm) column and a mobile phase constituted of acetonitrile, buffer pH 4.5 and methanol (55:40:5), flow rate was 0.8 ml/min. The method was validated according to the regulatory guidelines with respect to precision, accuracy, linearity and limit of detection (LOD) and Limit of Quantitation (LOQ). The percentage RSD for precision and accuracy of the method was found to be less than 2%.The method was found to be linear from 10-50 ppm for Olmesartan.

Keywords: Olmesarton, HPLC, validated.


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