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Abstract

EVALUATION OF ANTIBIOTIC PRODUCTION IN MICROMONOSPORA BY HPTLC

Padma Singh and Garima Sinha*

ABSTRACT

A total of 5 different types of actinobacteria were isolated from the soil
of Ganges river bed, Haridwar. All the isolated actinobacteria were
characterized and identified based on the morphological and
biochemical characteristics. The isolates were belonged to the genera
Micromonospora, Streptomyces and Nocardia. Only Micromonospora
sp. was selected for further studies. Antibacterial potential was
determined by both cross streak method and disc diffusion method.
The result of the screening revealed that the crude extract was effective
against E.coli and S. aureus. It was further analyzed by high
performance thin layer chromatography. Rf value in the TLC
chromatogram characterized that, the metabolite comes under
aminoglycoside group very much related to gentamicin.

Keywords: Micromonospora, E. coli, S. aureus, Bacillus, antibiotic production, HPTLC.


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