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Abstract

EVALUATION OF PHYTOCHEMICAL AND ANTIBACTERIAL EFFECT OF V.NEGUNDO AGAINST FOOT INFECTED CLINICAL PATHOGENS

Abdul Kapure M* and S. Ahmed John

ABSTRACT

The aim of the present study was to evaluate the antibacterial effect of leaves of Vitex negundo against wound infecting pathogens. 75 patients satisfying criteria of diabetic foot infection randomly Selected and divided in to three age group (A,B,C). Clinical isolates were isolated from foot infection and identified based on biochemical characters. Their sensitivity pattern against V.negundo extract was performed by stroke method and the active fraction was identified by TLC. among the three groups, group B between 40-40 years showed maximum prevalence of gram positive S.aureus infection with 57.6 % methicilin resistant. Nearly 106 colonies were isolated and belongs to eight different genera namely Staphylococcus aureus, Escherichia coli, Proteus vulgaris, Proteus mirabilis, Enterococcus faecalis, Enterobacter sp, Acinetobacter sp, Pseudomonas aeruginosa. All these isolated strains were highly sensitive to hexane extract of V.negundo and the active fraction nature was identified as flavanoid.

Keywords: V.negundo, Foot infection, Methicilin, Flavanoid, S.aureus.


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