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Abstract

COMPARATIVE EVALUATION OF NATURAL MEDIA FOR THE CULTIVATION OF B.SUBTILIS IN CONTINUOUS FERMENTATION

Ranjith Kumar P*, Manjula S, Sateesh B And Kiran B

ABSTRACT

Evaluation of population of B.subtilis on dry coconut cake, cotton seed cake, groundnut seed cake and nutrient broth in continuous fermentation was analyzed for 6, 12, 18, 24, 30, 36, 42, 48, 54, 60, 66, 72, 78, 84, 90, 96, 102 and 108 hours of fermentation and the maximum and continuous growth was observed in cotton seed cake broth and recorded 160 x108 CFU in 108 hours of duration. Cotton cake broth was followed by coconut cake extract and showed a significant growth in 60 to 78 hours of fermentation and recorded 130x 108 CFU. But the population deceased from 84 to 108 hours of fermentation. Groundnut seed cake extract recorded a significant growth at 78 to 84 hours and decreased from 90 hours of fermentation. All the result was compared with standard nutrient broth and recorded a highly significant activity of 160x108 CFU in 78 and 84 hours of fermentation.

Keywords: Coconut seed cake, Cotton seed cake, Groundnut seed cake, Nutrient broth, B.subtilis.


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