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Abstract

INVESTIGATION OF ANTI-HIV, CYTOTOXICITY AND HEPATOPROTECTIVE ACTIVITIES OF MORINDA CITRIFOLIA L (NONI)

Paul Pandi T*, Selvam P and Rama Mohan GupthaV

ABSTRACT

In recent years all other forms of immunodeficiency syndrome have been overshadowed by an epidemic of severe immunodeficiency caused by a retrovirus called Human immunodeficiency Virus type 1 or HIV-1. Numerous medicinal plants and their formulation are used for liver disorders in ethanomedical practices and in traditional system of medicine. Morinda citrifolia (Noni) commonly known as Indian mulberry and enriched with Flavonoids, anthraquinone, steroids, glycosides its fruits has been widely used in tropical regions both as food and folk medicine. Plants are reported to have a broad range of therapeutic effects including antibacterial, anti-viral, anti-tumor, analgesic, anti-inflammatory and immune enhancing properties. Objective: Acetone, Ethanol, Methanol extracts and isolated compounds were tested for their Cytotoxicity activity against Hep G2 cell, Hepatoprotective activity against Chang liver cells and anti-HIV activity against replication of HIV-1 (IIIB) and HIV-2 (ROD) in MT-4 cells. Methodology: Dried Noni fruit powder was subjected to successive extraction by using Soxhlet extractor with Acetone (MCF-Ac), Ethanol (MCF-Et) & Methanol (MCF-Me) for 72 hours. Noni fruit extracts were tested for Cytotoxicity effect against HepG2 (Human Liver Cancer) cell culture by MTT assay. Ethanol & Methanol extracts were further subjected to Silica gel-column chromatography to identify the active components. Noni extracts and isolated compounds were screened for Hepatoprotection on Human liver derived Chang liver cells against CCl4 induced damage by using MTT reduction assay and anti-HIV activity against the replication of HIV-1 (IIIB) and HIV-2 (ROD) in MT-4 cells. Results: The results revealed that the MCF-Ac, MCF-Et, MCF-Me extracts showed cytotoxicity against HepG2 cells with CTC50 values of 200 μg/ml, 220 μg/ml & 246 μg/ml. MCF-Ac and MCF-Me extracts treated with different concentrations and showed a dose dependent increase in Hepatoprotection percentage ranged between 72 – 84% at 100 – 150 μg/ml. MCF-Ac, MCF-Et and isolated compounds from MCF-Me extracts (C-9, Me- I, II & III) were tested against anti-HIV, MCF-Ac extract exhibited anti-HIV-1 & 2 replication in MT-4 cells with maximum protection 2 - 76% IC50 values of 157.0 and 37.04 μg/ml, respectively. Conclusion: This result strongly supports the basis for the use of extracts and isolated compounds were exhibits significant cytotoxicity activity against Human liver cancer cells. Acetone and Methanol extracts showed promising hepatoprotective activity against Chang liver cells and Acetone extract of Noni fruit exhibit anti viral activity against replication of HIV-1 (IIIB) in MT-4 cells.

Keywords: Morinda citrifolia Fruit, Anti-HIV, Cytotoxicity, Hepatoprotective activity.


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