COMPARATIVE TOXICITY ASSESSMENT OF ORGANOCHLORINE AND DIMETHOATE ON SERUM PROTEIN AND AMINO ACID PROFILE OF SWISS ALBINO MICE MUS MUSCULUS AND RELATED HEPATIC ANOMALIES: IN VIVO STUDY
Prakriti Verma* and G. B. Chand
ABSTRACT
Swiss Albino mice Mus musculus was exposed to [email protected]
mg/kg. body wt., rogor @20 mg / Kg. body wt. separately & a
combination of endosulfan(@1 mg /kg. body wt) and rogor (@10 mg/
Kg .body wt.) for 7, 4 & 21 days respectively. At the termination of
each exposure, blood sample were collected & serum was extracted to
analyze the quantitative estimation of total protein and amino acid
composition by standard method of Moore & Stein using thin layer
chromatography. The hepatic tissues were processed for light
microscopic studies. The results showed an increasing trend of serum
protein content at all the exposure level of rogor while a decreasing
trend was reported against endosulfan exposure. An abrupt trend of fluctuation in serum
protein was reported in combined exposure. Amino acid profile of control group showed a
very high percentage of valine, serine, aspartic acid, tyrosine & glutamic acid while a very
diminished concentration of these amino acids were found in the serum of treated groups.
The hepatic tissues showed corresponding anomalies in different treated groups. Hence
abrupt fluctuation in serum protein, diminished amino acid contents can be considered as
sensitive indices of toxicity generated by agrochemicals in mice.
Keywords: Mus musculus, Endosulfan, Rogor, Hepatic tissues, Light microscopy, TLC, Total protein, Amino acid.
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