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Abstract

ISOLATION OF ENDOPHYTIC FUNGI FROM LEAVES OF AZADIRACHTA INDICA AND PRELIMINARY SCREENING FOR ANTIMICROBIAL ACTIVITY

Pranay Jain* and Tarun Kumar

ABSTRACT

Endophytes are microorganisms that reside asymptomatically in the
tissues of higher plants and promising source of novel organic natural
metabolites exhibiting a variety of biological activities. The Objective
of present study was to isolate endophytic fungal strains from the
leaves of Azadirachta indica and screen the isolated strains for
antibacterial and antifungal metabolites production. The leaves were
sterilized, inoculated in the potato dextrose agar medium and incubated
at 28±2˚C for 7days. A total of 4 strains TK1, TK2, TK3, and TK4
were isolated and used for further studies. The strains were cultivated
on potato dextrose broth in 500 ml Erlenmeyer flask containing 200 ml
medium and incubated in BOD shaking incubator for 10-12 days at
28±2°C with periodic shaking at 150 rpm. Resulting broth was
separated from the mycelium and extracted 2-3 times with ethyl acetate
to obtain crude extracts. Agar well diffusion method was used to evaluate the antimicrobial
potential of the fungal isolates. Out of these four strains TK3 identified as Aspergillus sp.
showed antimicrobial activity against five Gram positive bacteria (Streptococcus pyogenes,
Streptococcus mutans, Staphylococcus aureus, Bacillus subtilis, and Bacillus megaterium),
two Gram negative bacteria (Escherichia coli and Pseudomonas fluorescens) and five fungal
pathogens (Candida tropicalis, Candida glabrata, Candida albicans, Alternaria solani and
Fusarium graminearum). Zone of growth inhibition against test pathogens ranged between
16-36 mm. The results showed the ability of the isolated fungal strain TK3 capable of
producing antimicrobial compounds and helped in justifying the traditional use of
Azadirachta indica against human pathogenic bacteria.

Keywords: Asymptomatic, Antifungal, Antibacterial, Endophytes, Azadirachta indica.


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