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Abstract

IN VITRO ANTIMICROBIAL EFFICACY OF DATURA INNOXIA LEAF EXTRACT AGAINST GRAM POSITIVE BACTERIA

Surendra Singh Parihar*, Rajnarayan Gupta, Priyanka Rana, Rajesh Singh Tomar

ABSTRACT

Datura innoxia leaves were examined for potential antibacterial activity by preparing their crude aqueous and Ethanol extracts against Gram-positive bacteria (Bacillu subtilis and Staphylococcus aureus). The results of Disc diffusion method indicated that the pattern of inhibition depends upon the plants part, solvent used for extraction and the organism tested. Extracts prepared from leaves were shown to have better efficacy. Ethanol extracts provided potent antibacterial activity as compared to aqueous extracts. Ethanolic extract showed the antibacterial activity against gram positive bacteria. Gram-positive bacteria Bacillus subtilis were found most sensitive as compared to Staphylococcus aureus. This study concluded that Datura innoxia leaves may present a potentially active antibacterial agent in near future.

Keywords: Datura innoxia, Antibacterial activity, Drug resistance, Sterile Disc, Minimum inhibitory concentration.


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