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Abstract

FLOW CYTOMETRIC ANALYSIS OF IMMUNOADJUVANT ACTIVITY OF EMBLICA OFFICINALIS ON HUMAN WHOLE BLOOD

Amit Gupta and Sushama R Chaphalkar*

ABSTRACT

In this study, alkaloid fraction from Emblica officinalis was evaluated for its potential ability as an adjuvant effect on the immune responses to hepatitis and diphtheria-pertussis-tetanus (DPT) antigen on human whole blood using flow cytometry. Cells were treated with variable doses of alkaloid fraction (0.625 – 2.5 mg) in presence or absence of hepatitis and DPT vaccine antigen. Hepatitis and DTP vaccine containing alum used as standard for these studies. The results showed that the DPT mediated blood counts were significantly enhanced by alkaloid fraction at lower doses (0.625 mg) compared with DPT control group. Moreover, alkaloid containing hepatitis antigen showed no adjuvant effect on human whole blood.

Keywords: Emblica officinalis, hepatitis,


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