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Abstract

COMPARATIVE PHYTOCHEMICAL STUDY OF ROOTS VERSUS SMALL BRANCHES OF VITEX NEGUNDO L. USING HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHIC ULTRAVIOLET DETECTION METHOD

S. C. Verma*, E. Vashishth, S. Subhani, R. Singh, P. Pant, M. M. Padhi,A. Kumar

ABSTRACT

Vitex negundo L., family Verbenaceae is an important medicinal plant and immensely used in the Indian System of Medicine to cure human diseases. Roots of V. negundo have been reported for various medicinal properties such as relieving intermittent fever, thirst, body pain, to cure rheumatism, dyspepsia, piles and as an anthelmintic. V. negundo is commonly known as Nirgundi in India. Chemo-profiling screening of two parts of V. negundo plants revealed variations in phytochemicals within roots and small branches. The unique patterns of the chromatographic fingerprint were validated by analyzing roots and small branches of V. negundo. Our results revealed that the chromatographic fingerprint combined with similarity measurement could efficiently identify and distinguish V. negundo roots and small branches. The phytochemical fingerprint profiling of small branches and roots of V. negundo were found similar as an official part of V. negundo plant i.e. root, therefore small branches may be used in place of roots and vice-versa after comparison and confirmation of same pharmacological activities. The method can also be used for identification of different V. Negundo species and adulterants.

Keywords: V. negundo L., HPTLC–UV detection, phytochemical fingerprint profiling analysis.


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