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Abstract

TERATOGENETIC EFFECTS OF IGR, LUFENURON ON THE EMBRYONIC DEVELOPMENT OF GALLUS DOMESTICUS

Wagh Pinakin, Salokhe S.G.* and Deshpande S.G.

ABSTRACT

Chick embryos Hamburger Hamilton (HH) stage 4,5 cultured by New’s single ring technique when treated with insect growth regulator (IGR), Lufenuron at concentration of 54 ppm with different doses showed severe teratogenesis. At the dose of 10ul of 54ppm Lufenuron embryonic development is normal but it lags behind to that of control embryo. At 100ul dose of 54ppm Lufenuron teratogenetic effects, such as neural tube defects, anophthalmia, cardiomegaly, fan shaped large somites and absence of somites were observed. Also, it affects the development of embryonic blood vessels. This indicates that IGRs exert their teratogenetic effect by targeting mesodermal and ectodermal tissues and key events in morphogenesis.

Keywords: New’s culture, Chick embryo, IGR, Lufenuron, HH staging, somites.


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