STUDY OF ANTIMICROBIAL PROPERTIES OF MEDICINAL PLANTS BY AGAR WELL DIFFUSION METHOD
Sonia Chaman*, Shalini, Garima Sharma, Anil K Reshi
Department of Biotechnology & Bioinformatics, Sri GuruGobind Singh College, Sec -26, Chandigarh,160019. India.
ABSTRACT
Antimicrobial activity of ethanolic leaf extracts of Cymbopogon
citratus, Elettaria cardamomum, Elaeocarpus ganitrus and Fortunella
japonicawas studied againstPseudomonas aeruginosa(MTCC No.424),
Staphylococcus aureus(MTCC No.87), Candida albicans (MTCC
No.1964)by agar well diffusion method. Elettaria cardamomum had
shown antimicrobial activity against Pseudomonas aeruginosa,
Candida albicans and Staphylococcus aureus (with zone of inhibition
20, 19 &16 mm, respectively). Cymbopogon citratus had shown
inhibitory effect against Candida albicans, Pseudomonas aeruginosa
and Staphylococcus aureus (with zone of inhibition 19, 18&8mm,
respectively). Elaeocarpus ganitrus had scored maximum inhibition
against Pseudomonas aeruginosa and Candida albicans (with zone of
inhibition 20 mm) and minimum against Staphylococcus aureus (with
zone of inhibition 15 mm). Among all the tested plants Fortunella japonicaexhibited
maximum antifungal activity (with zone of inhibition 23 mm)and minimum antibacterial
activity against Staphylococcus aureus (with zone of inhibition 8 mm).Antimicrobial activity
against selected strains varies among the different plants used.The results showed that the
selected plants exhibited maximum antifungal activity followed by antibacterial activity
against Gram negative and Gram positive bacteria.This study suggests that the ethanol leaf
extract of selected plants can be used as potent agent for the drug formulation against
Candida albicans, Pseudomonas aeruginosa and Staphylococcus aureus.
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