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Abstract

ISOLATION AND CHARACTERIZATION OF BACTERIAL MICROORGANISMS THAT ARE ASSOCIATED WITH “EGUSI” (MELON SEED) SOUP SPOILAGE

*Ikeyi Adachukwu P., Ogbonna Ann O., and Uche Modesta U.

Department of Science Laboratory Technology Institute of Management and Technology (IMT) Enugu, Nigeria.

ABSTRACT

Five samples of egusi (melon seed) soup collected from different food vendors in (Campus 3) of the Institute of Management and Technology, Enugu were studied for bacteria spoilage. Different media were prepared and they include: MacConkey agar medium, nutrient agar medium, and blood agar medium. After 24 hours of culturing on the media, observation of the plates incubated aerobically show heavy and profused growth of Bacillus cerus, moderate growth of Escherichia coli and Klebsiella aerogenes as well as scanty growth of Streptococcus feacalis and Staphylococcus aureus. The presence of E. Coli and Streptococcus feacalis indicated faecal contamination of the soup as these two organisms are known to be indicator organisms. They are usually associated with poor hygienic handling of foods, water, utensils and vegetables. Having known the spoilage organisms, the shelf life of egusi (melon seed) soup could be extended and improved by subjecting the soup to a more advanced preservation methods such as canning and drying.

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