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Abstract

DERIVATIVE SPECTROSCOPIC ESTIMATION OF ACIPIMOX IN BULK AND PHARMACEUTICAL DOSAGE FORM

K.Lurdhu Mary* and Dr. A.Elphine Prabhakar

ABSTRACT

Derivative spectrophotometry involves the conversion of a normal spectrum to its first, second or higher derivative spectrum. Derivative spectrophotometry is a useful means of resolving two overlapping spectra and eliminating matrix interferences or interferences due to an indistinct shoulder on side of an absorption band. There are first, second, third and fourth derivative spectrophotometric methods. In the present study Acipimox in bulk and pharmaceutical dosage form was estimated by Derivative spectroscopic method by using UV visible spectrophotometer with distilled water as a solvent. Absorption maxima was found o be 231nm.The developed method was validated for Linearity, Accuracy, Precision.Repeatability studies and Assay. The developed methods were simple, accurate, sensitive and precise and found to be within prescribed limits.

Keywords: Derivative Spectrophotometry, Acipimox, Accuracy and Precision.


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