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Abstract

EVALUATION OF GENOTYPE AND PHENOTYPE METHODS FOR DETECTION OF BIOFILM FORMED BY STAPHYLOCOCCUS EPIDERMIDIS ISOLATED FROM PATIENTS IN SOME HOSPITALS OF BAGHDAD CITY

Nihad Khalawe Tektook*, Rajwa Hassen Essa, Susan Saadi Hussein

ABSTRACT

Coagulase-negative staphylococci have important role as pathogen and
most common cause of bacteremia related to indwelling device, S.
epidermidis was the most frequently isolated species in blood cultures
and most important of the skin flora, it can cause severe infections after
penetrates anatomic barriers, because it produces various proteases,
peptidases, surface lipoproteins that promote host tissue adherence and
biofilms. The research included isolated 50 S. epidermidis strains from
clinical specimen (60% blood culture , 22% catheters urine , 12%
wound and burn swab as well as 6% skin and nasal swab from hospital
staff.) from 480 patients at ( Imam Ali ,Al-Kendy, Al-wasety, Ibn-Albalade and Baghdad )
Hospital, In a period of study ( February to July 2014) .Identified and diagnostic these
bacteria by APi 20, VIETK 2 system, as well as classical method. Slime producing which
detected by three phenotyping methods 60% of bacterial isolate were positive biofilm
produced by microtiter plate, while ( 38, 16)% respectively were positive for both tube and
CRA methods. While (34,20,10,2)% of the S. epidermidis isolates from blood culture, urine
catheters, wound and burn swab as well as swab of Skin and nasal hospital staff produced
biofilm phenotypically.The results indicated that the IcaA gene had the highest rate in blood
culture (34 %) and Catheter urine specimen (20%) followed by IcaD gene which was
detected in 38 % of blood culture and 20% of Catheter urine specimen. The prevalence of
IcaA gene (66%) was low versus that of IcaD gene (72%). 27, 28 strain of
staph.epidermidis were biofilm-positive for Ica A and Ica D respectively by Mtp while 15
strain were positive to both IcaA and IcaD by tube methods. Most positive both genotype (Ica A, Ica D) and biofilm produced Were 28(56%) while negative for both of them and negative biofilm produced were 13(26%), While 5(10%) were positive for Ica A / D and negative for phenotype (biofilm produce). In relation of S.epidermidis, 66% (29/50) of clinical strain present Ica genes and slime producing. While four only out of 50 clinical strain are producing slime but losing the Ica genes also 26% (13/50) of clinical strain were non-slime producing and Ica genes were negative.

Keywords: Staph. Epidermidis, biofilms, Ica(Intercellular adhesion).


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