WJPR Citation New

  All Since 2011
 Citation  2903  2393
 h-index  27  24
 i10-index  68  60

Login

Best Paper Awards

World Journal of Pharmaceutical Research (WJPR) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.
            Best Paper Award :
Dr. Muhammad Baqir MR Fakhrildin
Download Article: Click Here

Search

Track Your Article

Abstract

DEVELOPMENT AND VALIDATION OF METHOD FOR DETERMINATION OF LUTEIN BY HPLC

*Md. Habib Ullah Bhuyian, A.F.M. Ariful Islam, Md. Isha Tareque, Dr. Harun Ar Rashid

ABSTRACT

A simple selective and rapid reversed phase high performance liquid chromatographic (RP-HPLC) method for The analysis of Lutein 5% has been developed and validated. The separation was achieved from HPLC Column ( C18 250mm x 4.6 mm, 5 μm) with a mobile phase Acetonitrile, Methanol, Water and Ethyl Acetate in the ratio of 70:9·6:0.4:20. Flow rate 1.0 ml/min with UV-visible detection at 446 nm. The method is specific and it is observed that no interference with diluents. Proposed method is accurate with (99.89%-100.16%) recovery for Lutein. The proposed method was accurate, and precise for the quantification of Lutein 5%. The proposed can also be used for routine analysis in quality control. The method was validated for parameters like selectivity, sensitivity, precision, intermediate precision, accuracy, linearity, recovery & stability. This (RP-HPLC) method is suitable for determining the concentration of Lutein 5% and it can apply for routine analysis for determination of the Lutein from dosage form.

Keywords: HPLC Column, Carotenoids, Zeaxanthin FDA, ICH, ?-carotene.


[Full Text Article]

Call for Paper

World Journal of Pharmaceutical Research (WJPR)
Read More

Email & SMS Alert

World Journal of Pharmaceutical Research (WJPR)
Read More

Article Statistics

World Journal of Pharmaceutical Research (WJPR)
Read More

Online Submission

World Journal of Pharmaceutical Research (WJPR)
Read More