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Abstract

IDENTIFICATION AND APPRAISAL OF CRUDE PROTEIN EXTRACTS OF INDIAN MARINE EDIBLE OYSTER CRASSOSTREA GRYPHOIDES FOR POTENTIAL BACTERICIDAL PROPERTY

P. Swapna and T. Ravinder Reddy*

ABSTRACT

A wide range of bioactive substances are being isolated and characterized from the food that is derived from the marine environment, several with enormous assure for the treatment of various diseases. Marine organisms are a wealthy resource of structurally novel and biologically active metabolites. So far, frequent chemically exclusive compounds of marine origin with different biologically activity have been isolated and a number of them are under investigation and or are being developed as new pharmaceuticals. For the past two decades pharmaceutical industry has been relatively successful in overcoming problems due to single resistant determinants. In recent years, natural products from marine samples have a broad variety of biological activities and abundant therapeutic applications contain antiviral, antibacterial, antitumor activity and very different kinds of substances have been obtained. In general, the marine animals especially mollusks and their compounds constitute a practically unlimited resource of new active substances. Hence, the present study was carried out to determine the bactericidal activity of Crassostrea gryphoides protein against human pathogens. The edible Oyster Crassostrea gryphoides was collected from Kakinada seashore Andhra pradesh, India. Immediately it was extracted by using phosphate buffer at three different pH (4, 7 and 9) and all the extracts were screened against six different human pathogens such as Vibrio furnissii, V. carchariae, Salmonella bongori , Shigella sonnei, enterococcus faecalis and Aeromonas hydrophila by agar well diffusion assay. After 24 hrs of incubation the maximum inhibitory effect was observed against Vibrio carchariae, Enterococcus faecalis and Aeromonas hydrophila and the minimum inhibitory effect was observed against Vibriocho furnissii, Salmonella bongori and Shigella sonnei respectively. Whereas checking the minimal inhibitory concentration (MIC), the crude protein extract of Crassostrea gtyphoides was inhibited the bacterial strains with the minimum inhibitory concentration of not less than 0.1ml (100¼). The molecular weight of the crude protein was found from 11.2 to 72.2 kDa and the total protein content of phosphate buffer crude extract of Crassostrea gryphoides was found to be 312 ¼g/ mg. From, the results, the work has suggested to use this commercially available and protein rich (bactericidal) oyster in therapeutics for the development of novel antibiotics against multiple drug resistance (MDR) pathogenic microbes.

Keywords: Crassostrea gryphoides, pathogenic microbes. Therapeutics, novel antibiotics.


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