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Abstract

METHOD DEVELOPMENT AND VALIDATION OF STABILITY INDICATING RP-HPLC METHOD FOR SIMALTANEOUS ESTIMATION OF AZILSARTAN AND CHLORTHALIDONE IN PURE AND PHARMACEUTICAL DOSAGE FORM

N. Sunitha*, Subash C Marihal, T.Sai Sushma, A.Venu, B.V.Narasimha Rao, B.Appa Rao

ABSTRACT

A simple, accurate, precise method was developed for the simultaneous estimation of the Azilsartan and Chlorthalidone in Tablet dosage form. Retention times of Azilsartan and Chlorthalidon ewere found to be 2.876min and 3.652min. %RSD of the Azilsartan and Chlorthalidone were and found to be 0.28 and 0.47 respectively. %Recover was obtained as 100.08 and 100.18 for Azilsartan and Chlorthalidone respectively. LOD, LOQ values are obtained from regression equations of Azilsartan and Chlorthalidone were 0.49, 1.49 and 0.74, 2.23 respectively. Regression equation of azilsartan is y = 18208x + 2709 and of Chlorthalidone is y = 29300x + 6534. Regression co-efficient was 0.999. Retention times are decreased and that run time was decreased so the method developed was simple and economical that can be adopted in regular quality control test in industries.

Keywords: Azilsartan and Chlorthalidone, RP-HPLC, Validation.


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