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Abstract

EVALUATION OF THE PROTECTIVE EFFECT OF CHITOSAN IN MICE TREATED WITH CYCLOPHOSPHAMIDE USING GENOTOXIC ASSAYS AND BIOCHEMICAL MARKERS.

Inas S. Ghaly, Hasnaa A. Radwan, Amira Abd ElRaouf and Sahar S. Ahmed*

ABSTRACT

The protective effect of Chitosan in mice treated with Cyclophosphamide has been evaluated in this study using cytogenetic analysis, DNA fragmentation, biochemical markers and molecular genetic assay. The experimental animals divided into two groups as male and female, each group divided into four subgroups. Two subgroups received saline of 0.5ml/ animal as control. Two subgroups received a single i. p. injection of 50 mg/kg Cyclophosphamide in saline for 24 hours as positive control. Two subgroups received Chitosan low dose (15 mg/ kg) for 3 weeks followed by a single i. p. injection of 50 mg/kg Cyclophosphamide in saline for 24 hours. Two subgroups received Chitosan high dose (30 mg/ kg) for 3 weeks followed by a single i. p. injection of 50 mg/kg Cyclophosphamide in saline for 24 hours. The results of different parameters used confirmed that the treated subgroups (G♂2 and G♀2) with Cyclophosphamide induced significant different in the chromosomal aberration of bone marrow, the frequency of micronucleus, DNA damage% and all tested biochemical markers compared to the control and the other treated subgroups. The changes in RAPD markers between the control subgroups and treated Cyclophosphamide subgroups were obvious, including the changes in the polymorphic pattern and molecular size. The results of chromosomal aberration, the frequency of micronucleus, DNA fragmentation, biochemical markers analysis, and polymorphic pattern of RAPD markers in the treated male and female subgroups with Cyclophosphamide and Chitosan (low and high doses) showed a significant improvement compared to the treated Cyclophosphamide subgroups. The improvement increased toward the levels of the control subgroups in pretreated Chitosan at high dose. In conclusion, the results of this study suggested that the pretreatment with Chitosan at dose 30 mg/ kg could act as anti-mutagenic and chemo-protective agent against Cyclophosphamide mutagenic effect.

Keywords: Cytogenetic analysis, DNA fragmentation, Biochemical marker, Molecular genetic assay, Cyclophosphamide, Chitosan.


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