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Abstract

PH-SENSITIVE ALGINATE-BASED PARTICLE FOR CONTROLLED RELEASE OF DOXORUBICIN AGAINST BREAST CANCER CELLS IN VITRO

Myla Santiago*, Mark Kevin Devanadera, Jim Karl Villanueva, Anne Louise Ayson,Roy Vladimir Cagalingan, Angelica Bernadette Crisostomo, Aristea Bayquen

ABSTRACT

Alginate is a non-toxic, biodegradable, and naturally occurring polysaccharide found in marine brown algae. It has a long list of use in numerous drug delivery systems due to its biodegradability and biocompatibility. The study aimed to encapsulate doxorubicin using alginate via ionotropic gelation method and assess the capacity of encapsulated drug to kill breast cancer cells through cell viability assay. Formulation of alginate-based particles was done through ionotropic gelation method. Preliminary test include encapsulation of solution with pH indicators to determine the biocompatibility of alginate to doxorubicin. The results showed compatibility of calciumalginate with basic and acidic solutions. Encapsulation of doxorubicin in calcium-alginate based particles exceeded 90% encapsulation efficiency. The encapsulated drug manifests a controlled release profile of 7% in PBS pH 7.4 at 37C during the first hour. Delayed drug release is vital in drug delivery system. The capacity of the encapsulated doxorubicin against free doxorubicin to kill tumor cells was determined by an in vitro cell culture using breast cancer cells and tests its cell proliferative activity by MTT assay. Trypan Blue Assay showed the cell density upon delivery of the encapsulated drug decreased as time progressed. While the MTT assay showed that the encapsulated drug can inhibit the breast cancer cells proliferation with a 12.85%, 77.75% and 96.45% inhibition during 20, 40, and 60 hours respectively (p0.050). In that note, encapsulation of doxorubicin in a calcium alginate based particles are proven to be effective in terms of drug delivery, breast cancer cells viability and in minimizing chemoresistance of cells.

Keywords: Breast Cancer, Calcium Alginate, Ionotropic gelation, Doxorubicin, Encapsulation.


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