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Abstract

PHOTOPROTECTIVE ACTIVITY ETHANOLIC EXTRACTS AND CREAM FORMULATION OF CAMELLIA SINENSIS AND AZADIRACHTA INDICA

Hamid A. K.*, Shafiyah S., Shariq B., Jiyauddin K., M. Kaleemullah,
Samer A. D. and S. Budiasih

ABSTRACT

Ultraviolet (UV) rays can cause many diseases such as erythema and skin cancer when exposed in excess. Thus, it is important to protect oneself from UV rays by application of sunscreen. However, several issues were found regarding sunscreen effectiveness in previous studies causing researchers to study on plants as alternative sunscreen. Camellia sinensis (Tea) and Azadirachtaindica (Neem) have shown potential for its individual photo-protective activity in previous study but no literature have been found for both plants working synergistically. Therefore, this research aims to evaluate the photoprotective activity of C. sinensis and A. indica cream formulations with ratio of 1:1. Both plants’ leaves underwent soxhlet extraction separately and concentrated using rotary evaporator. The concentrated extracts were tested for their individual and combination of both plants antioxidant and in-vitro sun protective factor (SPF) activity using spectrophotometer. Oil-in-Water (O/W) formulations were made from the extract in tea to neem with ratio of 1:0, 0:1 and 1:1 and its SPF activity were tested. The SPF activity of the neem cream showed the highest SPF value of 1.32±0.15, followed by combination of tea neem cream (1:1) with SPF value of 1.00±0.21 and tea cream with SPF value of 0.93±0.00. With the significance of 0.143 (p<0.05), this research concluded that there is no significant synergistic photo-protective activity between C. sinensis and A. indica cream formulations.

Keywords: Camellia sinensis, Azadirachtaindica, Photo protective, SPF and O/W emulsion.


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