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Abstract

OPTIMIZATION OF ANTIMICROBIAL SUBSTANCE PRODUCTION BY STREPTOMYCES RW2-3 STRAIN ISOLATED FROM SEA WATER

Aruna V and Rajan S*

ABSTRACT

Aim: To optimize the process parameters for enhanced production of bioactive metabolites by Streptomyces RW2-3. Materials: Agar well diffusion assay was employed to study the effect of environmental parameters such as incubation period, pH, temperature and influence of various nutrients such as carbon and nitrogen source and minerals on the bioactive metabolites production by Streptomyces RW2-3. Results: Optimized and better antibacterial metabolite production was noted when the test actinobacteria was inoculated on M14 medium. The other components like maltose, beef extract and incoculum concentration was needed at 2% concentration. pH of the medium is adjusted with pH 7 and incubated for 7 days at 27C. As the strain exhibited potent antimicrobial activity it may be explored for biotechnological purposes. Conclusion: Result of the present study concluded that Streptomyces RW2-3 produced higher quantum of antimicrobial substances.

Keywords: Bioactive metabolite, Streptomyces, Optimization, antimicrobial substance


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