WJPR Citation New

  All Since 2011
 Citation  2903  2393
 h-index  27  24
 i10-index  68  60

Login

Best Paper Awards

World Journal of Pharmaceutical Research (WJPR) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.
            Best Paper Award :
Dr. Muhammad Baqir MR Fakhrildin
Download Article: Click Here

Search

Track Your Article

Abstract

DEVELOPMENT AND VALIDATION OF A RP-HPLC METHOD FOR THE SIMULTANEOUS DETERMINATION OF VITEXIN-2″-ORHAMNOSIDE AND HYPEROSIDE IN LEAVES AND FLOWERS OF HAWTHORN

Andriy Gudzenko*

State Laboratory for Quality Control of Medicines, State Institution “Institute of Pharmacology and Toxicology of National Medical Academy of Science of Ukraine”, Kiyv, Ukraine. 14, Eugene Potie str., 03680, Kyiv, Ukraine.

ABSTRACT

A method for separation and quantification of two flavonoids by reverse-phase high performance liquid chromatography (HPLC) was developed and validated. Flavonoids present in extract of the leaves with flowers of hawthorn (Crataegus pentagyna Waldst. et Kit) were analyzed. Vitexin-2″-O-rhamnoside and hyperoside were used as calibration standards. In this study, an excellent linearity was obtained with r higher than 0.99. Besides, the chromatographic peaks showed good resolution. With other validation data, including precision, specificity, accuracy and robustness, this method demonstrated good reliability and sensitivity, and can be conveniently used for the quantification of vitexin-2″-O-rhamnoside and hyperoside of the extract of the leaves with flowers of hawthorn. In summary, the method above can be considered specific, exact, precise, linear, robust and easy to perform. Further this method can be applied to a standardization of multicomponent herbal remedies, that incorporate leaves with flowers of hawthorn.

Keywords:


[Full Text Article]

Call for Paper

World Journal of Pharmaceutical Research (WJPR)
Read More

Email & SMS Alert

World Journal of Pharmaceutical Research (WJPR)
Read More

Article Statistics

World Journal of Pharmaceutical Research (WJPR)
Read More

Online Submission

World Journal of Pharmaceutical Research (WJPR)
Read More